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Clade support using 1,000 bootstrap replications was performed using the R function pvclust.
For visualization of the significant comparisons, heatmaps of the significant genes after FDR adjustment were produced with the heatmaps2 package in R. Hierarchical clustering of individual samples with 1000 bootstrap replications was performed with the R package pvclust [ 66] and heatmaps were sorted accordingly.
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To test the reliability of the classification, 500 bootstrap replications were performed.
A phylogenetic tree was constructed by the neighbor-joining method and 1,000 bootstrap replications were performed to evaluate the robustness.
Branch support was assessed with 1000 bootstrap replications, except for the combined analysis of all five RvhB6 duplicates (Figure S4), in which 500 bootstrap replications were performed, and the combined analysis (Figure 10), in which 1 million bootstrap replications were performed.
Bootstrap re-sampling was conducted stratified by gastroenterologist; 1,000 bootstrap replications were performed.
Trees were rooted at midpoint, and 1,000 bootstrap replications were performed.
The default parameters were used, except that 1000 bootstrap replications were performed.
In order to robustly and accurately estimate the parameters, a total of 10,000 bootstrap replications were performed.
One hundred bootstrap replications were performed to place confidence estimates on groups contained in the most parsimonious tree.
In addition, bootstrap analysis (1000 replications) was performed to obtain estimates of support for clades of the ITS trees.
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