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1,000 Bootstrap replicates were calculated with pairwise deletion and gamma distributed rates among sites (gamma shape parameter was 0.4729).
1,000 bootstrap replicates were calculated for both treeing methods.
One thousand bootstrap replicates were calculated to provide branch supports.
One hundred bootstrap replicates were calculated for each guidance alignment and sequences below 0.6 sequence score and 0.8 column score were automatically removed from the alignments.
One hundred bootstrap replicates were calculated on the resulting phylogenetic trees by using the Seqboot and Consense programs of the PHYLIP package.
For all analyses under the distance or maximum parsimony criterion, 1000 bootstrap replicates were calculated; for maximum likelihood, 500 bootstrap replicates were computed.
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Boostrap support (% of 1,000 bootstrap replicates) was calculated using the neighbor-joining, minimum evolution, and maximum parsimony methods.
Maximum likelihood trees and bootstrapping (1000 replicates) were calculated in PHYML [ 69].
18 The percentage of replicate trees in which the associated sequences cluster together in the bootstrap test (1000 replicates) were calculated, and branches with <50% bootstrap cutoff were collapsed.
Bootstrap statistics for 1,000 replicates were calculated by neighbor-joining analysis with a maximum composite likelihood model and a gamma parameter of 2, using MEGA version 4 (26 ).
Bootstrap support using 1000 replicates was calculated using MEGA version 4.0 [54.0
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