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Five hundred bootstrap replicates were applied to obtain consistent and reliable sequence divergence values.
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In an attempt to evaluate further alternative positionings of T. subtilis and R. contractilis, a procedure that randomly sampled among the 127 genes to construct 200 bootstrap replicates was applied.
The Maximum Composite Likelihood model implemented in MEGA 4.0 and bootstrap statistics (1,000 replicates) were applied to all the sites of the examined regions to build Neighbor-Joining trees (Tamura et al. 2007).
Bootstrap analysis (1000 replicates) was applied to evaluate the internal support of the tree topology.
Bootstrapping with 100 replicates was applied to determine the statistical support for each clade.
For construction of the phylogenetic tree, the Neighbor-Joining (NJ) method (JTT matrix with different rates among sites, gamma parameter = 1.0, bootstrap test = 1000 replicates) was applied on the MSA using MEGA (version 4.0) [ 68].
To test for nodal reliabilities, bootstrap (BS) analysis [ 50] for MP (1,000 replicates), ML (100 replicates) and NJ (1,000 replicates) was applied.
One hundred bootstrap replicates were computed and applied to the best scoring ML tree.
One thousand bootstrap replicates were run and the GTRGAMMA model was applied.
The bootstrap replicates were used to build a consensus tree applying the majority rule option (-m PROTGAMMAIJTTF -J MR).
Ten thousand bootstrap replicates were performed.
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