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To estimate the level of support, 1,000 bootstrap replicates were analysed using RaXML [ 99].
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1000 bootstrap replicates were performed for the NJ and MP analyses and 200 for the ML analyses, which are much more computer intensive.
A total of 1000 bootstrap replicates were performed for each maximum parsimony analyses.
For ML analyses 1000 bootstrap replicates were ran.
Bootstrap replicates were set to 1000 for all analyses.
One hundred and 1000 rapid bootstrap replicates were conducted for nuclear and plastid analyses, respectively.
In all the analyses, 1000 bootstrap replicates were performed to establish the reliability of the NJ and MP trees.
ML analyses (100 bootstrap replicates) were conducted using the Linux version of Phyml v2.4.4 [ 56], applying substitution models that were selected with ModelGenerator (gamma distribution with four rate categories)[ 57].
Distance analyses including 1500 bootstrap replicates were performed.
Default settings were used for the GARLI analyses and 100 bootstrap replicates were performed.
A GTR+G+I substitution model was used for all ML analyses, and 1000 bootstrap replicates were run to assess nodal support; models less complex than the GTR model are not implemented in RAxML.
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