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A bootstrapped phylogenetic tree (with 1000 bootstrap replicates) was constructed for the avian influenza H7 HA1 coding region using maximum likelihood inference in the PhyML software [ 90].
Sequence alignment was conducted using ClustalW, and a maximum-likelihood tree with 100 bootstrap replicates was constructed using MEGA version 4.0 (Tamura et al., 2007).
A maximum parsimony tree with branch confidence values based on 1000 bootstrap replicates was constructed.
A maximum parsimony tree with branch confidence values based on 500 bootstrap replicates was constructed and the tree drawn in TreeViewPPC version 1.6.6 –.
A neighbour-joining (NJ) tree based on 1000 bootstrap replicates was constructed by the Kimura model [ 79] using the TREECON 1.3b program [ 80].
A neighbour-joining tree following 1000 bootstrap replicates was constructed using MrBayes v3.22 [ 47] and plotted with FigTree (v1.4; http://tree.bio.ed.ac.uk/software/figtree/).ac.uk/software/figtree/
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Maximum-Likelihood based trees and 100 bootstrap replicates were constructed using PhyML [67].
Neighbour joining (NJ) phylogenetic trees, with 1000 bootstrap replicates, were constructed using MEGA5.2.2 [ 43].
ML topologies with 1,000 bootstrap replicates were constructed with PHYML 3.0 [ 45] and Bayesian phylogenetic reconstructions were carried out as previously described.
Neighbor-joining trees (with 500 bootstrap replicates) were constructed with PHYLIP [ 8] using amino acid sequences and simple Poisson-corrected substitution models (λ = 1).
For this, protein sequences alignment was performed using the MUSCLE program [ 77], and maximum likelihood trees with 100 bootstrap replicates were constructed with the PHYML program [ 78, 79] and the JTT amino acid substitution model.
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