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A 1000 bootstrap replicates was conducted with the preferred model in PAUP* 4.0b10.
To test for this possibility, each taxon was successively removed from the dataset (N = 63 experiments) and subsequently a likelihood run (using RAxML) under the GTR + 3 model with 1000 rapid bootstrap replicates was conducted for each resulting dataset.
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2,000 bootstrap replicates were conducted for each of these four datasets.
To corroborate the Bayesian results [108], maximum likelihood analyses of the codon alignments and maximum parsimony and neighbour-joining analyses of the aa alignments with 1000 bootstrap replicates were conducted using PAUP 4.0b10 [109].
200 bootstrap replicates were conducted.
GTRCAT and GTRGAMMA options were used, 1000 rapid bootstrap replicates were conducted to assess bootstrap values.
One hundred and 1000 rapid bootstrap replicates were conducted for nuclear and plastid analyses, respectively.
In addition, maximum likelihood analysis with 1000 and maximum parsimony analysis with 1000 bootstrap replicates were conducted.
1,000 bootstrap replicates were conducted on the data set using PAUP* 4.0b10 with maximum parsimony and using MEGA with neighbor-joining methods and the Poisson-corrected distance.
For analyses in BootScan, 1,000 bootstrap replicates were conducted under the Kimura model (transition/transversion ratio = 1.341), with a window size of 100 bp, step size of 20 nucleotides and cut-off value of 0.70.
ML analyses (100 bootstrap replicates) were conducted using the Linux version of Phyml v2.4.4 [ 56], applying substitution models that were selected with ModelGenerator (gamma distribution with four rate categories)[ 57].
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