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Branch supports were estimated from 1,000 PhyML bootstrap replicates using the same settings.
Based on the alignments, phylogenetic trees were constructed using the neighbor-joining method with 1000 bootstrap replicates using the MEGA 3.1 program.
Support for individual branches was assessed by conducting 100 non-parametric bootstrap replicates, using the rapid algorithm implemented in RAxML [111].
The phylogenetic tree was constructed by the neighbor-joining method with 1,000 bootstrap replicates, using the MEGA 4.1 software, and revealed that all of these sequences were grouped together in one cluster, but separate from other bocaviruses (Figure 1).
Phylogenetic trees were generated from homologies of the LKR/SDH amino acid sequences from different sources by the neighbor-joining method, and the confidence of the branching order was verified by making 1,000 bootstrap replicates using the fourth version of MEGA software.
RAxML was run with 1,000 bootstrap replicates using the GTRGAMMA model.
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Unrooted gene trees were reconstructed for the original MSA and each bootstrap replicate using the phylogenetic software ExaML [ 78].
1000 MP and 100 ML bootstrapping replicates using the same heuristic search parameters and model of sequence evolution were performed for each of the eighteen single copy PlantTribes examined, as well as the concatenated alignment of thirteen genes.
The super-alignments were used to build the consensus tree with 1000 bootstrap replicates using PROML (maximum-likelihood module) in the PHYLIP package [ 19].
Phylogenetic analysis was performed by the neighbor-joining (NJ) method [ 37, 38] and maximum parsimony (MP) method [ 38] with 2,000 bootstrap replicates using MEGA software [ 35] or the maximum likelihood (ML) method with 200 bootstrap replicates using PHYML [ 39] plugin for Geneious software http://www.geneious.com.
A phylogenetic analysis was performed with 1000 bootstrapping replicates using the neighbor-joining method in the tree-builder tool of the same software.
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