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A heuristic search with 10 random addition sequence replicates and 100 bootstrap replicates under the HKY model of sequence evolution [73] selected using ModelTest [74] was performed with and without constraining monophyly of Central American and South American individuals.
A maximum likelihood analysis using RaxML v7.0.3 [42], [42] with 1000 bootstrap replicates under the GTRCAT approximation was run on the combined dataset to check for congruence with the Bayesian analysis.
Confidence was inferred by running 1,000 bootstrap replicates under the GTRCAT model.
Maximum likelihood reconstruction was done using PhyML 3.0 [ 63, 64], with 1000 bootstrap replicates under the LG model.
Maximum-likelihood trees were constructed by using heuristic searches, and node support was assessed with 1,000 bootstrap replicates under the distance criterion with maximum-likelihood model settings.
The confidence of the branches of the best ML tree was further assessed based on 1000 rapid bootstrap replicates (under the GTRCAT model) (for more details see [ 21]).
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To better understand estimator variance, we plotted the gamma distributions specified by the shape parameter estimators inferred for each bootstrap replicate under the FL2 and FL3 models.
Phylogenetic inferences were performed by the neighbour-joining method with 1,000 bootstrap replicates under Kimura's two-parameter correction using MEGA 5.
Node support for each character set was gauged by performing 1000 bootstrap replicates under default search parameters.
Maximum likelihood analysis in this case was performed under the GTRGAMMA model, with the number of bootstrap replicates automatically determined under the recommended autoMRE option, and gaps treated as missing data.
The estimated area under the ROC curve (AUC) for death was 0.72 (95% CI; 0.57, 0.85 for 2000 bootstrap replicates) and the area under the ROC curve for revision was 0.49 (95% CI; 0.38, 0.62).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com