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This used 1000 bootstrap replicates from the matrix of the number of base substitutions per site based on the Maximum Composite Likelihood method [16].
Confidence intervals were estimated by taking 1,000 bootstrap replicates from the total pool of sequences.
The extreme outliers are the comparison of each of the bootstrap replicates from the concatenated dataset against the same set of 100 bootstrap replicates.
A maximum-likelihood tree was generated using RAxML [ 65] with 100 bootstrap replicates from the binary data based on the presence or absence of the genes (2,364 genes) in the accessory genome.
We calculated the weighted statistic that accounts for topological uncertainty by estimating the GSI for each group on each of the 100 randomly selected bootstrap replicates from the phylogenetic analyses.
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A 50% majority rule consensus tree of the 1000 bootstrap replicates from GARLI was then created using PAUP*.
To obtain 95% confidence intervals for the parameters, sites were sampled with replacement from the full data set to produce 10,000 bootstrap replicates from which parameters were calculated.
Phylogenetic trees were constructed with the neighbor-joining method with 1,000 bootstrap replicates from a matrix of Jones–Taylor Thornton distances in MEGA5 (Tamura et al. 2011) and using model-based Bayesian inference in MrBayes 3.2.2 (Ronquist and Huelsenbeck 2003).
This model was used to generate 100 bootstrap replicates from which a consensus tree was generated.
The Characters program produces a matrix with the original k-tuple distances (original file) and a second output comprising the bootstrap replicates calculated from the original matrix (bootstrap file).
This tree was used as the input tree to generate further full ML trees using the PhyML program [ 71] with 100 bootstrap replicates generated from the input alignment.
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