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For each alignment, two types of trees were constructed, a neighbor-joining (NJ) tree with 100 bootstrap replicas using the ClustalW software with default parameters (BLOSUM distance matrix) [ 69], and a maximum-likelihood (ML) tree using PhyML v3.0 with default parameters [ 70].
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Two types of trees were constructed: a neighbor-joining tree with 1000 bootstrap replicas using standard settings (Gonnet weight matrix, gap opening penalty 10.0 and gap extension penalty 0.20) in Clustal X 1.81 and a quartet-puzzling maximum likelihood tree using the Windows version of Treepuzzle 5.2 [23].
The standard deviation of the Bootstrap replicas was used as an estimate of the standard error of the classification images.
One thousand bootstrap replicas were used to calibrate the new models compared to 60 or 100 in Rosetta1, thus allowing the uni-variate and bi-variate probability distributions of predicted parameters to be quantified in greater detail.
We ran 100 bootstrap replicates using the rapid hill-climbing algorithm.
Bandwidth, which previously bootstrapped, will be using the capital for hiring and R&D.
Additionally, the reverse type replica mold was fabricated using the flexible replica mold.
Phylogenetic trees with bootstrap analysis from 1,000 replicas were generated by using the neighbor-joining method.
Phylogenetic trees with bootstrap analysis from 1,000 replicas were generated by using the neighbor-joining method as described previously (8 ).
Calculate the variation coefficients (CVs) intra-experiment using the replicas inside experiments and inter-experiments.
Strains containing multiple antibiotic-resistance genes were tested using the replica plating method.
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