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For each analysis, 100 bootstrap pseudoreplicates were conducted in Garli, and these were summarized on the ML tree with the program SumTrees v3.3.1, part of the DendroPy package [ 80].
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For both methods, bootstrap analyses (200 pseudoreplicates) were conducted to evaluate the statistical significance of the reconstructed trees.
1000 bootstrap pseudoreplicates were performed to assess support for clades.
For both methods of ML analysis, 1,000 bootstrap pseudoreplicates were used to assess the support for the ML topology.
For the ML analyses, full heuristic ML and bootstrap (200 pseudoreplicates) searches were conducted with 10 random addition replicates and tree bisection-reconnection branch swapping using the best-fit model in PAUP* 4.0b10 [55].
ML bootstrap analyses were conducted with the same settings described above (100 pseudoreplicates; one heuristic search per pseudoreplicate).
Bootstrap inferences were conducted separately for each loci with 500 pseudoreplicates.
Nodal support of the ML tree was estimated using bootstrap method [ 47] with 100 pseudoreplicates; Maximum parsimony (MP) analyses were conducted using heuristic search with 100 random sequence additions and tree-bisection-reconnection (TBR) branch swapping.
To assess levels of support for different nodes in the tree, bootstrap analysis was conducted with 1,000 pseudoreplicates.
ML analysis was performed using PHYML 3.0[ 43] with a BIONJ starting tree under the GTR model and nonparametric bootstrap analysis was conducted with 500 pseudoreplicates.
Bootstrap analysis was conducted by evaluating 1,000 pseudoreplicates of the alignment with SEAVIEW program 4.2.12 [ 39] using the neighbor-joining method.
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