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These two hypothetical groups appear to be more closely related to alkaline ceramidases (with a modest bootstrap percentage value of 82% for grouping them with alkaline ceramidases) than to the other known groups according to the phylogenetic tree.
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The numbers at the nodes represent bootstrap percentage value based on 1,000 replications.
In each of the phylogenetic tree, bootstrap percentage values that showed a stability of at least 80% were considered.
Bootstrap percentage values greater than 50% are given at the nodes of the tree (1000 replicates).
Consistent with the CLANS grouping, each of the eleven groups in Figure 1 is monophyletic in the MOLPHY phylogenetic tree, generally with good statistical support (nine out of the eleven groups with bootstrap percentage values above 80%).
Bootstrap percentage values are shown in Additional file 4: Figure S2.
Bootstrap percentage values (50%) generated from 1,000 replicates from maximum parsimony and posterior probabilities (>80%) from Bayesian analysis are shown as [Maximum parsimony bootstrap value/Bayesian posterior probabilities].
Phylogenetic trees were constructed by using the neighbor-joining method; data were resampled 1,000× to generate bootstrap percentage values.
In the context of resampling plans, n is the number of replicates and p is the bootstrap or jackknife support value (bootstrap percentage or bootstrap p value) expressed as a fraction of 1, i.e., the proportion of replicates that yielded a tree containing a particular phylogenetic group.
Support values for main branches are given as percentages of bootstrap values of bootstrap values in 1000 pseudoreplicates.
For bootstrap support levels, we considered bootstrap percentages (BP) of 50 70% as weak, 71 85% as moderate and >85% as strong (Kores et al. 2001).
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