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A total of 35 cycles were run.
All test cycles were run at 20°C.
A total of 45 cycles were ran in the qPCR.
Two independent runs with 5,000 bootstrap cycles were calculated, starting with a random tree.
The number of required bootstrap cycles was determined with the –autoMRE command (Stamatakis 2014).
100 bootstrap replicates were run and bootstrapping frequencies (BF) were calculated.
One thousand bootstrap replicates were run and the GTRGAMMA model was applied.
In the ML analysis, 1,000 bootstrap replicates were run to assess support for specific nodes.
500 bootstrap replicates were run to estimate branch support.
100 bootstrap replications were run in GARLI v0.951.0sX-GUI [ 42] under the same parameters.
Therefore, in addition to parametric analyses, non-parametric bootstrap methods were run in Stata [ 37].
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