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As shown in figure 5, the PlusC OBPs clustered as seven major phylogenetic clusters based on bootstrap cutoff value of 50%, but we further subdivided them into 11 subtypes (mplus1– mplus11).
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The input for the "Vote" algorithm includes the set of bootstrap trees (bTree1,... bTree n ), the "family defining bootstrap cutoff values" which are derived from well studied known families and are tree specific, and a "evaluation bootstrap cutoff value" which is used to evaluate families defined in other trees.
This analysis indicates that selecting bootstrap cutoff values lower than the recommended default setting of 50% results in considerable enhancement of sensitivity, with little reduction of specificity (especially for RIO).
The family defining bootstrap cutoff values are tree-specific, and are set to be the smallest bootstrap value of the reference miRNA families (let7, mir-124, mir-17 and mir-1, See additional file 3: Reference miRNA families) in each input tree.
ASaturA analyses were performed using PAM250, Kimura's correction and a cutoff value of 9, with 1000 rounds of bootstrap.
Phylogenies were constructed for all datasets with the Neighbor-joining algorithm, 1000 bootstrap repetitions, p-distance estimations as a statistical model, uniform substitution rates and a partial sequence cutoff value of 95%%.
FDR cutoff value of 0.05 was used.
A typical cutoff value of E < 1.0-5 was used.
The optimal cutoff value of the NLR was 2.17.
Generally, a cutoff value of |3| is considered statistically significant.
The cutoff value of IgG by ELISA was 0.26.
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