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The unrooted bootstrap consensus tree generated from sequence alignment of the recA gene sequence, coupled with the neighbor-joining method, suggested homology between F. tularensis ssp.
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A neighbor-joining cluster was generated, with bootstrap values calculated by resampling 1000 times, and a consensus tree generated, all using default parameters in Mega 5.04.
A bootstrap consensus tree was generated to assess the phylogenetic support of the branching [ 14].
In both analyses, a bootstrap consensus tree was generated from the 100 resulting consensus trees using the CONSENSE program of the PHYLIP package.
A bootstrap consensus tree was generated from 100 replicates [ 47] and branches corresponding to partitions replicated in less than 70%% replicates were collapsed.
The bootstrap consensus tree was subsequently generated by the CONSENSE option of the PHYLIP package [ 65].
Only bootstrap values >50 % and common to the consensus trees generated either by Winboot and UPGMA are shown (Felsenstein 1985).
A bootstrap consensus tree from this analysis was then generated and drawn with MEGA4 [ 48].
Each of the 100 resulting consensus trees was then used as an input for the program Consense of the Phylip package to generate the bootstrap consensus tree [ 65].
Programs from the PHYLIP 3.69 package were used to create pseudoreplicate datasets (SEQBOOT), compute distance matrix (PROTDIST), calculate distance trees (NEIGHBOR), and generate the bootstrap consensus tree (CONSENSE).
A bootstrap consensus tree, whose topology was identical to the best tree, was generated from the 100 resulting consensus trees using the CONSENSE program of the PHYLIP package.
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