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A phylogenetic analysis revealed that all Drosophila loci form a well-supported monophyletic clade with a bootstrap (confidence) value of 99%.
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To build phylogenetic tree, the maximum parsimony [ 26] method calculating bootstrap confidence values of 100 bootstrapping trials, using the MEGA 5.2 software, was used.
The tree identified a number of sequence clusters that were well resolved, with ≥ 95% bootstrap confidence values for the nodes.
Bootstrap confidence values were calculated on 1,000 replicates according to the maximum likelihood approach of Felsenstein (20 ).
The bootstrap confidence values were based on 1000 replicates.
Numbers represent bootstrap confidence values.
ML bootstrap confidence values were based on a 1000 replications.
Bootstrap confidence values were obtained for 1000 replicates.
Bootstrap confidence values greater than 50% are shown at branches.
Bootstrap confidence values were calculated for 1,000 pseudoreplicates.
The phylogenetic tree separated extracellular (apoCr, apoLp-II/I, apoB, Vtg, and CP) from intracellular (MTP family) LLTP sequences with confidence into separate clusters, suggesting that these extracellular proteins arose from a common ancestor (hereafter, support bootstrap confidence level values of NJ: 96 and ML: 88, and posterior probability of BI: 0.80).
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