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Unaligned, sequences in the equilibrated dataset were given taxonomic assignments at a bootstrap confidence range of ≥95% using the Ribosomal Database Project's Naïve Bayesian Classifier tool (RDP classifier) [25], [35].
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The sample average fitness of infected females was 0.61% lower than uninfected female, but the 95% bootstrap confidence interval ranged from 3.21% lower fitness to 2.03% higher fitness.
However, the uncertainty is relatively high, with a 95%% bootstrap confidence interval ranging from about 1percentto10percentcent.
To assess the sampling variability of the estimated accuracy, we used the standard 95% bootstrap confidence intervals, defined by the ranges between the 2.5th and the 97.5th percentiles.
Fig. 4 Principal stress directions at the spatial grid within the 95%% confidence range by the bootstrap test.
This is consistent with population genetics theory that predicts neutral variants linked to deleterious alleles should be found at lower frequencies [ 41]. 95% bootstrap confidence intervals of mean allele frequency ranged from 0.3802 to 0.4076.
The range between the outer and inner circles indicates that the values within the 95%% confidence range have been estimated by the bootstrap test described below.
DiCiccio, T. J. & Efron, B. Bootstrap confidence intervals.
Values in parentheses are 95% nonparametric bootstrap confidence intervals.
The shaded regions represent 95% bootstrap confidence intervals.
Fig. 6 95% bootstrap confidence intervals for global weights.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com