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A bootstrap analysis was preformed on the phylogenetic network [see additional file 5].
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Statistical analysis was preformed with SAS 9.2 (Cary, NC) for Windows.
To confirm that ASL3 was the gene associated with the phenotype observed, RNA interference (RNAi) analysis was preformed.
Statistical analysis was preformed using SPSS 13.0 for MicroSoft™ Windows.
All slides were kept at 4°C in dark until confocal microscopy analysis was preformed.
Statistical analysis was preformed using student's t-test (SPSS. Chicago, IL).
Univariate analysis was preformed, separately, on each morphometric parameter, using ANOVA (analysis of variance), with STATISTICA software.
TLC analysis was preformed as in ref (49).
The analysis was preformed using Sigmastat 3.5 (Systat Software, San Jose, CA, USA).
Sulfation analysis was preformed as described previously [ 22].
Gels were scanned and data analysis was preformed by the Bioinformatic Section of Genedata, Basel, Switzerland.
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