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One hundred replicates for bootstrap analyses were run in RAxML and PHYML, and a 50% majority rule consensus was calculated to determine the support values for each node.
Rapid bootstrap analyses were run in RAxML-VI-HPC v2.0.1 [ 105, 106] via the CIPRES Science Gateway [ 107] applying partitioning for each gene region using a GTR + CAT approximation rate substitution model and the rapid Bootstrap algorithm with 100 replicates [ 106].
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5%to90%10%(10% intervals between 10% and 50%, 5% intervals between 55% and 90%) of the fastest evolving sites were then removed (percentage of the total rate distribution), and bootstrapped ML analyses were run with each of these 14 shorter alignments.
In order to assess the robustness of models and predictors, bootstrap and permutation analyses were run with 100,000 permutations for some of the above models (see results in Figure 3).
Analyses were run as the rapid bootstrap procedure (option –f a) with bootstraps defined by option –NautoMR.
ML analyses were run under the TIMef + Γ model with 1000 bootstrap replicates in Garli v. 2.0 [ 79] implemented in the Cipres Science Gateway [ 80].
All analyses were run in triplicate.
Two independent analyses were run in parallel.
All analyses were run in duplicates.
All analyses were run in JMP7 (SAS Institute).
Analyses were run using SPSS.
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