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Therefore, serum collected from mice primed and boosted with the recombinant proteins were tested for HAI titers.
Indeed, same vaccine boosted with the recombinant vaccinia virus encoding HPV16/18 E6/E7 fusion protein TA-HPV with or without imiquimod is now utilized in another clinical trial started in 2008 with expected results in 2016 [ 24].
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Inclusion of a eukaryotic secretion signal or boosting with the recombinant protein resulted in higher specific-antibody levels and protection.
We further show that boosting with the recombinant Ab2-vβ proteins generates B and T cell memory response specific for antigen in anti-id DNA primed mice.
In this study, the mycobacterial antigen HSP65 was used to determine whether priming cattle with a plasmid DNA vaccine and subsequently boosting with the recombinant protein in adjuvant (heterologous prime-boost approach) would result in improved and more homogenous immune responses over immunising with plasmid DNA or protein in adjuvant alone.
The secretory IgA level was significantly increased in the mucosa of mice immunized orally with the Ts87 DNA vaccine, either boosted with the same DNA (DNA + DNA) or with the recombinant protein (DNA + P) compared to the group with the protein prime-boost (P + P).
They then boosted with the adenovirus/HIV vaccines.
All animals boosted with the same amount six weeks later.
We found that mice immunized with vaccinia, primed with Gag plasmid DNA, and boosted with a recombinant vaccinia virus lacking the majority of the B5 ectodomain expressing a test antigen, HIV Gag, had stronger anti-Gag immune responses than mice that were boosted with a wild-type virus-expressing Gag.
For example, Ramshaw et al. showed that mice primed with DNA encoding HA and boosted with a recombinant fowlpox virus produced extremely high levels of anti-HA serum antibodies, predominantly of the IgG2a isotype, and were protected against homologous influenza virus challenge [42].
Rabbit anti-murine jagged-1 and anti-delta-like 4 antibody were prepared by multiple-site immunization of New Zealand White rabbits with recombinant protein (R&D Systems, Rochester, MN) in CFA and boosted with recombinant protein in IFA as in previously described procedures from our laboratory [7].
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