Accordingly, after immunization with the antigen in CFA and boosting with the antigen in IFA, the plasma aAbs levels of all mice (i.e., both groups in both batches) were significantly elevated, compared to the baseline condition (Timing, F(1.95) = 11,12, p <0.05).
Thus, mice vaccinated first with a mixture of purified N1 and N2 proteins and subsequently boosted with the individual antigens showed a small memory response also against the heterologous subtype (40).
Three weeks after the first injection, the rabbit was boosted with the same amount of antigen mixed with complete Freund's adjuvant.
The mean frequency observed with iPEM capsules (3.1%) represented a 4.5-fold enhancement over the level (0.7%) observed in mice treated and boosted with the admixed formulations of antigen and polyIC.
Rabbits were given a primary s.c. immunization with 250 μg of recombinant protein MAR3-MBP emulsified 1 1 in Freund's complete adjuvant and were subsequently boosted with the same dose of antigen emulsified 1 1 in Freund's incomplete adjuvant at 4-week intervals.
Both ARC elicited a strong and lasting primary antibody response, and remarkably in the absence of caldarchaeols in their lipid compositions, an enhanced memory humoral response after boosting with the bare antigen in C3H/HeN mice upon subcutaneous immunization.
Upon subcutaneous immunization of C3H/HeN mice, BSA entrapped in ARC-BM or ARC-GC elicited a strong and sustained primary antibody response, as well as improved specific humoral immunity after boosting with the bare antigen.
In group 4, three pigs (numbers 12 to 14) were vaccinated with BEI-inactivated C-S8p260 with adjuvant, and boosted with the same dose and composition of antigen at 15 days post-immunization.
injection on day 0. The mice were boosted with the same preparation on day 7. Sham-immunized (SI) mice were given similar injections but without the antigen (mBSA).
We confirmed their observation of autoantibody responses following immunization and boosting with the same peptide antigen and extended the work by immunizing with a peptide from rabbit N-methyl-D-aspartate receptor NR2b (GR) [5].
