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We hypothesized that, after the first neonatal injection, the degeneration of muscle to fibrocalcific/fibroadipous tissue was prevented, thus a late injection with a different serotype could result in a transduction of the surviving muscle and boost the expression of the delta-sarcoglycan.
With an approach reciprocal to miRNA inhibition, the use of miRNA mimics represents an attractive tool to boost the expression of downregulated miRNAs.
Importantly, we found evidence to suggest that this approach could be readily adapted to boost the expression of any recombinant cytosolic or membrane-bound protein.
Fusing the protein to MBP, which helped to boost the expression of AldO significantly (Heuts et al. 2007), did not have any visible effects on HotAldO expression levels.
However, in some special cases where miRNAs are globally reduced such as the blockage of their biogenesis from Dicer knock-down or gene mutations [ 19], this normalization would artificially boost the expression of reduced miRNAs and obscure true differences.
Further, MLL4 is required both to allow cells to specialize into different cell types, and to boost the expression of genes that are specific to each type of mature cells.
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They turned this theory into reality by boosting the expression of the genes responsible and crucially showed this worked in agricultural fields, with a 15% increase in productivity.
Active NtrC binds to DNA and boosts the expression of certain genes that allow the cell to live without nitrogen.
These should be directed at the level of gene selection and delivery, in order to identify the optimal cytokine and achieve efficient and durable cytokine expression, and at the level of improving immune stimulation, i.e. by co-administration of co-stimulatory molecules including B7 and CD40, or boosting the expression of tumor antigens or MHC class I molecules.
Recently, Rose et al. have demonstrated that signals responsible for boosting the expression are most abundant in introns near the transcription start site and that the compositional difference between promoter proximal introns and distal introns can be used to predict the ability of an intron to stimulate expression [21].
Instead, selection may operate at functional level, for example, in boosting the expression of AMPs [ 13].
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