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In a second animal, Ag85A/b-specific (0.18%) CD4+ T cells could be detected one week after the first boost (Figure 4A) and TB10.4-specific (0.4%) CD8αβ+ T cells were detectable four weeks after the second boost (Figure 4B).
However, BALB/c mice did show a modest increase in domain 4-specific IgG after the boost (Figure 1B).
Potentially conformational anti-mimotope responses (as represented by the V3 and KLIC groups) were not boosted by the last protein boost (Figure S3C; data for KLIC not shown).
ELISA titers to HA, NP and M2 were determined after DNA immunization (Figure 4, white bars) and after the rAd5 boost (Figure 4, black bars).
A single animal showed Ag85A/b-specific (0.35%) and TB10.4-specific (0.19%) CD4+ T cells, one week after the first boost (Figure 4A).
One animal showed Ag85A/b-specific CD4+ T cells (0.25%), and two animals exhibited TB10.4-specific CD8αβ+ T cells (0.3 and 0.42%) one week after the first boost (Figure 4A, B).
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