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Molecular approaches can serve to boost expression levels and target the expressed protein for appropriate post-translational modification.
Gene synthesis enables the exploitation of the degeneracy of the genetic code to boost expression of recombinant protein targets for structural studies.
To boost expression, a new expression vector was designed and constructed, and Fab59 light chain codons were optimized for bacterial expression.
It is possible that, because of the increased length of the mRNA in plants transformed with the double protein construct, the mRNA becomes more efficiently translated than in the case of the smaller mRNA in plants transformed with the single-protein construct, indicating that the polyprotein expression strategy might boost expression levels of small proteins.
Alternatively, codon-optimized genes may be utilized to boost expression in such cells without the need for a second antibiotic.
To boost expression, transfections were modified as follows: 1) increasing the amount of mutant α12.2 and α2δ1 plasmids to 3 µg, 2) adding 0.5 µg of plasmid containing the SV40 T antigen, and 3) incubating the cells 48 hours before plating onto chips.
Similar(50)
Each of A11, A17 and P7 appeared to have a dominant "poisoning" segment; replacement of that segment with the corresponding segment from a good expresser boosted expression close to the level seen in the good expresser.
Fortunes have been spent acquiring new players since the unfortunate Champions League final last May, yet the one attribute that stands above any quantity of skill or boosted expression of ability is the simple change in ethos within the team and among the players.
A new study pins this airy achievement on a bone growth gene, whose boosted expression may have literally helped bats sprout wings and take off.
Specifically, IFN-γ production by mucosal T and NK cells retards disease resolution by maintaining signaling through the transcriptional regulator STAT1 and boosting expression of inflammatory mediators like IL-1β, TNF, and iNOS.
This evidence is in line with a recent finding in plants that indicates that the signals responsible for boosting expression are more abundant in proximal than in distal introns [21].
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