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We previously reported the development of DNA-C and MVA-C vaccines based on HIV-1 subtype C and demonstrated their immunogenicity when given in a DNA prime-MVA boost combination in a nonhuman primate model.
Ad-H4, rH4, the hetrologous prime boost combination, non-vaccinated, and BCG vaccinated mice were included.
In contrast, the heterologous prime boost combination resulted in augmentation of both the CD4 and CD8 response.
The other track of malaria vaccine development is genetic vaccination with DNA plasmids, recombinant viral vectors, or often with both in a DNA prime-viral boost combination.
We clearly show that rH4/CAF01 induces primarily CD4 T cells, and Ad-H4 CD8 T cells, but the heterologous prime boost combination induces both CD4 and CD8 T cells.
Currently, a focused Phase II study is evaluating the VRC HIV-1 DNA prime - rAd5 boost combination for its effect on early control of viral load in those study participants who become HIV-1 infected.
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To test this proposition, we have generated gene-based vaccine vectors expressing Ag85B from Mycobacterium tuberculosis (Mtb) and designed experiments to test their immunogenicity and protective efficacy particularly when given in heterologous prime-boost combination, with the initial DNA vaccine component given soon after birth.
Newcastle disease virus (NDV) expressing HIV-1 BaL gp160 was evaluated either alone or with monomeric BaL gp120 and BaL SOSIP gp140 protein in a prime-boost combination in guinea pigs to enhance envelope (Env -specific humoral and mucosal immunEnv -specific
The 6-plasmid DNA/rAd5 vector prime-boost combination has recently been evaluated in larger multicenter, international studies.
All subjects underwent apheresis before and after rAd5 boosting to characterize in depth the T cell and antibody response induced by the heterologous DNA/rAd5 prime-boost combination.
These vectors were initially chosen based on reports of improved cellular immunogenicity when used in a prime-boost combination in humans with a variety of antigens [7] [9] and on their ability to control viremia after multiple routes of SHIV challenge in rhesus macaques [10], [11].
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