Tissue specimens (soft tissue and/or bone) were obtained in lieu of swab cultures.
Four bone measurements namely mesiodistal distance, cortical bone thickness, buccolingual thickness, and bone density were obtained in the axial slice sections corresponding to the various heights from the arch wire within the limits of mucogingival junction (Figure 1c and Figure 2).
For the analysis of trabecular bone, measurements were obtained in an area of the distal epiphysis of the femur spanning from 75 µm from the growth plate to the endocortical edge of the epiphysis, as previously described [48].
Tumor-infiltrating bone marrow samples were obtained in 27 patients at baseline and in 30 patients at any time point.
For the remaining 37 subjects, separate measurements of air conduction in each ear (0.25 8 kHz), and unmasked bone conduction (0.5 4 kHz), were obtained (in one subject with normal hearing, bone conduction was not obtained but was assumed equal to air conduction in the better hearing ear).
For analysis of Piezo1-tdTomato expression and splenic RBC composition, single cell suspensions of blood, bone marrow, or spleen were obtained in PBS containing 2% FBS.
For trabecular bone parameters, transverse CT slices were obtained in the region of interest in the axial direction from the trabecular bone 0.1 mm below the growth plate (bottom of the primary spongiosa) to the mid-femur.
Diagnostic bone marrow or tissue samples were obtained in 20 ALL, 20 AML, 11 CML in chronic phase, 50 CLL, 55 MM and 74 NHL patients.
Similar data were obtained in bone marrow-derived MSC [ 14, 27].
Similar observations were obtained in bone marrow chimeric mice where JNK1 signaling was disrupted in hematopoietic cells [ 73].
