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Bones were mounted in OCT (Tissue-Tek) and snap frozen.
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Bone tissue samples were mounted in a staining solution with 0.17 mm thick coverslips.
Stained bone-marrow cells were mounted in VECTASHIELD mounting medium with DAPI Vector Laboratoriess, Burlingame, CA, USA) or Prolong Gold (Invitrogen) and observed under a BZ-9000 fluorescence microscope (Keyence Corporation, Osaka, Japan) or a FluoView FV10i confocal laser scanning microscopy (Olympus).
The bone-plate constructs were mounted in a servo-hydraulic testing machine (Bionix 858, MTS Systems, Minneapolis, USA; Figure 2) and tested at axial compression using 100 loading-relaxation cycles per testing level.
To elucidate osteoclastogenesis the bones were decalcified in 10% EDTA in PBS pH 7.4.The decalcified femurs were mounted in the cryo-sectioning mounting media (Electron Microscopy Sciences, Hatfield, PA) and stored at −80°C before sectioning.
Four others were mounted in 1934.
Slides were mounted in Vectashield mounting medium (Vector Laboratories).
Leaf epidermal slides were mounted in DePeX mounting media.
Cells were mounted in the holding device.
Six of these were mounted in casemates.
They were mounted in six Dop.
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