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Initially, the facial bones were detached from the neuro-cranium.
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Culture expanded bone marrow-derived MSCs were detached from culture plates using trypsin-EDTA and formed a 5×106 cells/mL suspension.
Differentiated bone marrow-derived macrophages (BMDMØ) were detached from Petri dishes by incubation with 0.01 M phosphate-buffered saline (PBS) pH 7.2 supplemented with 20 mM Hepes and 1% EDTA for 20 min.
Up to three giant OC with more than 25 nuclei/cell were observed per slide and most of them were detached from the bone surface [Figure 1].
Modified SCL was performed as previously described; 6 sternohyoid muscles of both sides were detached from the hyoid bone, leaving inferior vascularization.
Gels were detached from the plates.
Cells were detached from the culture flasks using versene (Invitrogen).
Cells were detached from confluent 100-mm dishes.
After 30 60 minutes, all cells were detached from the substratum.
Before cutting the bone block, the CAL is detached from the CP medially by careful subperiosteal preparation of the lateral two-thirds of the CP.
From then on, Peterson played with a tendon in his throwing arm that was detached from the bone.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com