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A dissecting microscope (AXS, Anxi Optical Equipment Manufacturing Co, Shanghai, China) was used to ensure that the articular cartilage and subchondral bone were isolated.
In order to determine if SK-Mel-29 CM has any effect on mature osteoclast activity, osteoclastic giant cells from two specimens of fresh giant cell tumour of bone were isolated by digestion with collagenase as previously described (Lau et al, 2005) and cultured in the presence and absence of SK-Mel-29 CM.
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Working on the 3D-Volume Rendering Reconstruction, the fractured bone was isolated with digital scissor tool.
Also, the tumor and the bone are isolated from each other.
The proximal tibial bone was isolated, and soft tissue was removed to a maximal extent, without harming cartilage integrity.
When in the ground, the bones were isolated by anoxic moist clay that prevented this from happening, but when removed into the drier open air, the natural chemical conversion began to occur.
Parietal bones were isolated and cleaned from the adherent connective tissue.
Later, selected bones were isolated: right femur, right tibia, and L4 vertebra.
On day 7, 24 hours after the last injection, blood samples were collected and the long bones were isolated.
The temporal bones were isolated and placed in 4% formaldehyde overnight after opening the round and oval windows.
Briefly, bones were isolated and flushed with high glucose Dulbecco's modified Eagle's medium (DMEM; 10 % fetal bovine serum and 0.5%% penicillin/streptomycin) [ 36].
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