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On the right, a Nodosaurus tibia (shin) that later became a home for oysters when the bone was washed out to sea.
After digestion, cancellous bone was washed with DPBS and transferred to cell culture flasks in culture medium (MEM/Ham's F12, 10% FCS, 2 mM L-glutamine, 100 U/ml penicillin, 100 µg/ml Streptomycin, 50 µM L-ascorbate-2-phosphate, 50 µM β-glycerol-phosphate).
After defatting in methanol and chloroform (1 1) the bone was washed in distilled water and cut under constant irrigation into slices of 10 or 1 mm thickness.
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Specimens that included the full thickness cartilage and subchondral bone were washed with sterile PBS and the macroscopic cartilage degeneration was determined by staining with India ink [ 19].
Cartilage samples, excluding the mineralized cartilage and the subchondral bone, were washed twice in PBS (BioWhittaker, Verviers, Belgium) and then minced.
Explants including epiphyseal cartilage and about 5 mm of the soft underlying bone (considered as subchondral bone) were washed in PBS and placed in DMEM culture medium for subsequent mechanical-loading experiments or flash frozen in liquid nitrogen for subsequent RNA-seq analysis.
The bones are washed with awamori and placed in huge family sarcophagi that reminded me of gigantic turtle shells.
It is likely the bones were washed from a nearby burial site along one of London's "lost" rivers - the Walbrook.
The crushed bones were washed in HBSS+ (Gibco) supplemented with 2% FBS, 10 mM HEPES, and 1% Penicillin/Streptomycin(P/S) to remove the hematopoietic cells.
The remaining diaphy-seal bones were washed with phosphate-buffered saline (PBS) to remove all marrow elements and blood cells.
Decalcified bones were washed in tap water for 16 hours, dehydrated in ethanol/toluene, and embedded in paraffin.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com