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The hole bone was sealed using bone wax, and rats were given a dose of enrofloxacin (10 mg/kg) during 48 hours.
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After a 2-min delay to allow cells to fill the bone cavity, the syringe was removed and the drill hole was sealed using bone wax.
The keyhole was sealed using bone wax, and the skin was closed using size 1 sutures.
Subsequently, the bony calvarium was sealed using a droplet of bone wax to prevent reflux, and the skin was sutured.
Finally, the hole was sealed using a Ti foil.
At last, the borehole was sealed using Bimbar-4 seals and high-pressure liquid was injected into the groove.
Each dish was sealed using a coverslip and vacuum grease.
To prevent drying, the plate was sealed using parafilm.
Burr holes were sealed using bone wax and the scalp was sutured closed.
Caps of the crucibles were sealed using asbestos strings.
Coverslips were sealed using Cytoseal 60.
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