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Gravity Bone was developed using a modified version of id Software's id Tech 2, the graphics engine for Quake 2. Chung acknowledged that although he has worked with newer, "powerful and flexible" engines, he preferred the older engine because it was released as an open source platform, "so you can redistribute it for free".
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Therefore, an adequate and safe animal model for bone augmentation was developed using a standardized femoral and tibia augmentation site in sheep.
A novel porous three-dimensional bone scaffold was developed using a natural polymer (alginate/Alg) in combination with a naturally obtained biomineral (nano cockle shell powder/nCP) through lyophilization techniques.
A kind of novel three-dimensional scaffolds for bone tissue engineering was developed using the biomimetic synthesis method combined with sol gel and freeze-drying techniques.
Peroxidase reaction was developed using ECL.
More recently, animal models of human bone metastasis have been developed using subcutaneous implantation of human bone but these models suffer from problems such as donor-related variability and poor viability of the implant.
Composite systems for bone replacement have been developed using various bioactive ceramic or glass reinforcements in a bioinert polymer matrix.
Toward this goal, silk-based scaffolds resembling bone lamellar structure were developed using a freeze-drying technique.
In this study, ceramic scaffold fabrication techniques for potential use in load-bearing bone repairs have been developed using naturally derived silk from Bombyx mori.
To overcome the limitation of autograft periosteum, synthetic tissue-engineered periostea have been developed, using osteoinduced rabbit bone marrow mesenchymal stem cells (MSCs) and porcine small intestinal submucosa (SIS), or using an acellular human dermis, which is capable of supplying cells and osteoinductive proteins.
Successful strategies for tolerance induction have also been developed using immunotoxin without the adjunct of donor bone marrow or stem cell infusion.
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