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As in a previous study (Samara et al. 2013), the relatively healthy bone was collected as control from the healthy region of the femoral headneck about 1 cm away from the zone of osteonecrosis in each patient (in the no-ON group).
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The bone from the anterior and lateral walls of the sinus was collected as part of the antrostomy.
The supernatant was collected as nuclear fraction.
Supernatant was collected as soluble fraction.
Tibialis and femur bone marrows were collected as a positive control for BrdU incorporation by flushing the tibia and femur with a 28 gauge needle.
During surgery, cancellous bone samples were collected, as described below.
Murine bone marrow cells were collected as previously described.
Fresh trabecular bone was collected from patients with and without osteoporotic fracture with homogenous anthropometric parameters such as age, BMI, gender or disorders affecting bone remodelling.
Other tissues, such as bone (femur), skin and heart, were collected as quickly as possible.
Bone marrow and peripheral blood samples were collected as part of initial diagnostic investigations.
The trabecular bone from the laminar and ILC that were excess to the surgical requirement were collected as biopsies of size approximately 0.25 to 1 cm3.
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