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Total bone volume was calculated for the entire pellet.
A cylinder (width: 5.5 mm, height: 3.5 mm) was selected in the metaphysis of the tibia (Fig 1B, region 3), containing only trabecular bone, of which bone volume was calculated.
The change in percentage bone volume was calculated by dividing the difference in experimental and control defect BV/TV values by the control BV/TV value and then multiplying by 100.
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Quantitative measurements of bone surface and bone volume were calculated for each metatarsal bone.
After capture and reconstruction of three-dimensional radiographic images, the BV/TV index, defined by the ratio between the volume of bone tissue (mineralized) and total sample volume, was calculated in the samples with high bone formation (scores 3 and 4) based on the histological analysis.
Then the images were taken at 1-mm increments mesio-distally, and the volume was calculated by adding the entire area of the bone grafting material.
Tumor volume was calculated using the formula width2 × length × 0.5.
Tumour volume was calculated according to the formula: tumour volume=length × width2/2.
Tumor volume was calculated using the ellipsoid formula: volume = 1/2 × a × b2 (a = length, b = width).
Tumor volume was calculated every week using the formula (volume = length × width2/2).
Flap volume was calculated using dedicated software.
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