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Similarly, the capacity of osteoblastic cells to perceive pericellular oxygen has a profound effect on skeletal mass and architecture, as mice expressing stable hypoxia-inducible factor (HIF -1α and -2α demonstrate age-dependent increases in bone volume per tissue volume and osteoblast number.
For quantitative histomorphometric analyses, we measured bone volume per tissue volume (BV/TV) and osteoblast surface per bone surface (Ob.S/BS) of rabbit bones in MM-bearing SCID-rab mice as the indices of bone destruction and osteoblast function, respectively.
Bone volume per tissue volume (BV/TV), osteoblast surface per bone surface (Ob.S/BS) and tumor per tissue volume (tumor/TV) were measured in decalcified implanted rabbit bone sections stained with H&E as the indices of bone destruction, osteoblast function and a tumor burden, respectively, as described previously.
Parameters like fractional bone volume (bone volume per tissue volume, BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th).
Vessel density (vessel volume per tissue volume, VV/TV) was computed in analogy to bone volume per tissue volume (BV/TV).
Micro-CT assessment showed a 35% decrease of bone volume per tissue volume (BV/TV) in vehicle-treated hTNFtg mice between week 8 and week 11 (figure 2A).
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Nonetheless, bone-volume-per-tissue-volume did not deviate statistically (was 17.6% versus 15.4% in the control group).
Histomorphometric examination of the femur supported these density measurements, with bone volume per total volume increasing by 32% (p = 0.04).
However, overall bone volume per total volume (%BV/TV) and bone mineral density (BMD) at the distal femur were comparable between the groups (Table 2).
The percentage of bone volume in tissue volume (%BV/TV), tumor volume in tissue volume (%TuV/TV), trabecular thickness in μm (Tb.Th)., trabecular number per mm (Tb.N ., trabecular separation in μm (Tb.Sp ., the ratio of osteoblast perimeter to bone perimeter as a ratio (Ob.Pm./B.Pm)., and the ratio of osteoclast number to bone surface (N.Oc./BS) were determined.
Abbreviations: %BV/TV, percentage bone volume in tissue volume; %TuV/TV, tumor volume in tissue volume as a percentage; Tb.Th., trabecular thickness in μm; Tb.N., trabecular number per mm; Tb.Sp., trabecular seperation in μm; Ob.Pm./B.Pm., osteoblast perimeter to bone perimeter; N.Oc./BS, ratio of osteoclast number to bone surface.
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