Histomorphometric examination of the femur supported these density measurements, with bone volume per total volume increasing by 32% (p = 0.04).
Trabecular bone volume per total volume (BV/TV%) was decreased in OVX and PbOVX animals in 54% compared to the control animals (p < 0.001).
For quantitative histomorphometric analyses, we measured bone volume per tissue volume (BV/TV) and osteoblast surface per bone surface (Ob.S/BS) of rabbit bones in MM-bearing SCID-rab mice as the indices of bone destruction and osteoblast function, respectively.
Bone volume per tissue volume (BV/TV), osteoblast surface per bone surface (Ob.S/BS) and tumor per tissue volume (tumor/TV) were measured in decalcified implanted rabbit bone sections stained with H&E as the indices of bone destruction, osteoblast function and a tumor burden, respectively, as described previously.
Parameters like fractional bone volume (bone volume per tissue volume, BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th).
Vessel density (vessel volume per tissue volume, VV/TV) was computed in analogy to bone volume per tissue volume (BV/TV).
However, overall bone volume per total volume (%BV/TV) and bone mineral density (BMD) at the distal femur were comparable between the groups (Table 2).
MicroCT was used to measure bone volume per tissue volume and bone density in a cylindrical volume comprising the implant, with settings as previously described (volume diameter: 2 mm; Agholme 2014).
Nonetheless, bone-volume-per-tissue-volume did not deviate statistically (was 17.6% versus 15.4% in the control group).
Mean bone volumes per total volumes were 55.7 ± 3.0% in the induction group, and 49.2 ± 3.2% in the control group (n.s).. Mean trabecular thickness was 0.119 ± 0.017 mm and 0.095 ± 0.010 mm, respectively (n.s).. Supplementary Figure 3 shows an example of a 3D reconstruction of the scanned area of a tibia from the induction group.
