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Leptin-deficient mice have significantly shorter femora, lower femoral bone mineral content (BMC), bone mineral density (BMD), cortical thickness, and trabecular bone volume compared to lean mice.
In addition, at the late time point only implants with the 200/800 pattern revealed a significantly higher bone volume compared to the rough controls.
In contrast to the pattern observed in the femur, ob/ob mice have significantly increased vertebral length, lumbar BMC, lumbar BMD, and trabecular bone volume compared to lean controls.
The same phenotype was observed in male transgenic mice, where we also found a more than 4-fold reduction of the trabecular bone volume compared to wildtype littermates at 24 weeks of age (data not shown).
Based on our findings obtained in the Col1a1-Krm2 transgenic mice we have now expanded the analysis of Krm2-deficient mice to 24 weeks of age and observed a marked increase of the trabecular bone volume compared to wildtype littermates, which is caused by a more than three-fold increase of bone formation.
The low dose SB431542 group showed a 47% reduction in bone volume compared to the BMP-only control group (P = 0.06).
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Histomorphometric analysis of bone in the proximal tibia after 6 weeks showed both Pb and HFD to be associated with a significant decrease in trabecular bone volume compared with LFD mice and significantly interacted to lower BV/TV.
μCT analysis of Terc −/− and Wrn −/−Terc −/− mice showed that they recapitulate this phenotype by having both a significant decrease in cortical thickness and a significant increase in porosity, which together contributed to the significant loss of cortical bone volume compared with WT mice (Table 1).
Results: The injection of EP4 agonist alone did not induce notable changes in body weight, macro-structures, or bone volume compared with the vehicle-treated group or the untouched control side.
The region we measured as a periarticular site was composed of a small bone volume compared with the whole skeletal bone, including the cortical bone compartment.
Overall, therefore, Thra1 PV /+ mice have increased cortical and trabecular bone volume compared with WT, but their bone is less mineralized.
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