Treatment with Zol dramatically improved bone volume, trabecular number and trabecular thickness compared to the Sham and MM groups.
Regarding cortical bone, the calorie-restricted groups had reduced values of bone volume, mean polar moment of inertia, and cortical thickness compared to the AL groups (P <.05).
In contrast, indices of bone formation were visible in 12-week LRHF and HRLF rats had several indices of net bone resorption in serum and in distal radial trabecular region, including increased osteoblast numbers; increased osteocalcin (a key serum biomarker of bone formation), compared to FRC, LRLF and HRHF rats; and increased trabecular thickness, compared to NC, LRLF and HRLF rats.
Interestingly, 12-week HRHF rats also showed increased trabecular thickness, compared to NC, LRLF and HRLF rats, indicative of some bone adaptation in this group.
The role of SIRT1 in bone remodeling was further confirmed in whole-body SIRT1KO mice showing reduced cortical bone thickness compared with wild-type mice (Fig. 4F).
Glass foams both with and without PRP showed an increase of bone thickness and histological bone formation compared to controls (no biomaterial).
Leptin-deficient mice have significantly shorter femora, lower femoral bone mineral content (BMC), bone mineral density (BMD), cortical thickness, and trabecular bone volume compared to lean mice.
As shown in Figure 5A and 5B, arthritic joints treated using medium and high doses showed lower grades of inflammation, synovial membrane thickness, and cartilage and bone destruction compared to those with control protein treatment or treatment with a low dose of immunotoxin.
MicroCT analysis revealed that male Nur77 KO mice had a low bone mass compared to WT male littermate controls, illustrated by a 36% lower bone volume/tissue volume ratio (BV/TV), 19% less bone surface (BS), 8% greater bone volume/bone surface ratio (BV/BS), 14% less trabecular number (Tb.N), 8% less trabecular thickness (Tb.Th), and 19% more trabecular separation (Tb.Sp).
RNFL thickness maps were compared to GDx (Carl Zeiss Meditec) thickness maps.
