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For analysis of actin rings, bone slices were fixed with 4% ultra-pure paraformaldehyde (Polysciences) and 0.2% Triton X-100 for 10 minutes, then washed with PBS 3 times.
After the culture period, bone slices were fixed as described above.
After the culture period, bone slices were fixed as described earlier above.
The cells were allowed to grow for 48 h, after which the bone slices were fixed with freshly made refrigerated 3% paraformaldehyde (PFA) and 2% sucrose in phosphate-buffered saline (PBS) for 10 minutes at room temperature.
Similar(56)
After culture, ivory slices were fixed in 4% paraformaldehyde and TRAP stained to identify osteoclasts.
Subsequently, slices were fixed overnight in 2% paraformaldehyde at 4°C.
At the end of treatment, slices were fixed in 4% PFA.
At the end of each recording, slices were fixed overnight in 4% paraformaldehyde.
After recording, brain slices were fixed with 2% paraformaldehyde and 1% glutaraldehyde and histochemically stained.
To this end, slices were fixed by immersion in 4% paraformaldehyde overnight.
Slices were fixed after recording in 4% paraformaldehyde (PFA) in 0.1 M PBS overnight.
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