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Table 1 SEM-EDS analysis for bone slice and cell mineralization on 3D woven scaffold made from PLA/HA fibers.
Pretreatment of the bone slice with dilute solutions of ammonium fluoride was found to result in greater resistance to osteoclastic resorption.
The resulting Ca/P atomic ratios were 1.54 and 1.35 for the bone slice and examined mineralization area, respectively (Table 1).
The microelectrode technique enables real-time data to be obtained, whereas the conventional method requires a long (ca. 16 20 h) incubation of the cells on the bone slice.
The OCT block containing the bone was then reversed and the procedure was repeated on the opposite face until a thick bone slice with bilaterally and evenly exposed BM content was obtained.
The case study used MRI to generate a model of each dancer's muscles, cartilage, and bone, slice by slice.
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For comparison, human bone slices were also analyzed using EDS.
Thirty-seven human thoracolumbar vertebral bone slices were prepared by removing cortical endplates and posterior elements.
A ham counter features domestic and imported varieties on the bone, sliced to order.
Afterward, bits of charred bone, slices of liver and even parts of the tree were sold as souvenirs.
Once BM slices were generated, the remaining OCT medium was removed by incubation and washing of the bone slices in PBS 3 times for 5 min.
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