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For static histomorphometry, decalcified implanted bone sections were stained with hematoxylin and eosin in most experiments; in some experiments, undecalcified bone sections were stained with Masson's trichrome and used for analysis.
Bone sections were stained with von Kossa/van Giseon reagent, images were captured using a Nikon eclipse 80i microscope and analyzed using NIS Elements AR 2.30 software (Nikon).
To localize the calvarial bone, sections were stained with alizarin red-S to identify areas of active extracellular mineral deposition indicating the presence of differentiated osteoblasts.
To confirm that differentiation was unaffected in mutant chondrocytes, long bone sections were stained for p57, a cyclin-dependent protein kinase inhibitor that is expressed by prehypertrophic, postmitotic chondrocytes [23].
Mineralized bone was visualized in non-decalcified, plastic-embedded sections using the von Kossa stain, and decalcified, paraffin embedded bone sections were stained with hematoxylin and eosin, using standard histological procedures.
Bone sections were stained with toluidine blue stain.
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For histologic evaluation of inflammation and bone erosion, sections were stained with either H&E or tartrate-resistant acid phosphatase (TRAP).
Femoral bone and sternum sections were stained with Giemsa solution.
To determine bone resorption parameters, sections were stained for tartrate-resistant acid phosphatase (TRAP).
Bone and spleen sections were stained using hematoxylin and eosin for visualization of tissue structure, or used for immunohistochemistry with antibodies to visualize specific cells: CD20, CD68 (Dako, Glostrup, Denmark) and CD3 (Leica, Wetzlar, Germany).
A microtome (Microm, Micron Microtech, Francheville, France, HM350S) was used to cut 7 9- μm thick sections of undecalcified long bones, and the sections were stained with Goldner's trichrome.
More suggestions(15)
bone sections were cut
bone erosions were stained
bone sections were captured
bone sections were removed
bone tissues were stained
bone slices were stained
bone samples were stained
bone sections were mounted
bone sections were assessed
bone sections were used
bone sections were visualized
bone sections were imaged
bone nodules were stained
bone sections were deparaffinized
bone sections were processed
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