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The undecalcified bone sections were produced using a thin-section technique according to Donath and Breuner [ 30].
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Both, thin sections and polished sections were produced to observe eosauropterygian bone microanatomy and histology, generally by removing a thin slice of bone with a rock saw.
Paraffin sections were produced as previously described [33].
10 μm sections were produced and used for subsequent staining.
Bone sections were stained with toluidine blue stain.
Finally the bone sections were stained with toluidine blue.
Bone sections were stained with hematoxylin and eosin (H&E) to visualize the bone morphology and submitted to histomorphometric analysis.
Transverse bone sections were made of the femur with a special bone saw (Exakt System, Norderstedt, Germany).
For histological analyses, paraffin sections of bones were produced from E18.5, p0, and p20 mice.
Coronal sections (50 μm) were produced using a Vibratome sectioning instrument (Ted Pella, Redding, CA, USA).
At least three thin sections per specimen were produced.
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