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Lung and bone sections were processed as previously described [ 6, 7].
Briefly, the bone sections were processed for antigen retrieval by digestion in 0.05% trypsin (pH 7.8) for 15 min at 37 °C, and then incubated with antibodies against LRP6 (diluted 1 50), osteocalcin (Ser463/465) (diluted 1 100) and osterix (diluted 1 400) overnight at 4 °C.
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Lungs, bones, and tumor sections were processed as previously described [ 8, 9].
Brain sections were processed for immunohistochemistry to identify β-amyloid deposits and GFP expressing bone marrow-derived microglia in the brain.
Sections were processed as previously described [23].
Sections were processed for immunohistochemistry as follows.
Paraffin sections were processed similarly as mouse liver sections.
Sections were processed for immunocytochemistry and histochemistry.
Tissue sections were processed as described above.
Ultrathin sections were processed for immunogold technique.
Then sections were processed as described above.
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