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To detect the hyaluronan expression, distal femur bone sections were deparaffinized, rehydrated, and incubated with 3% hydrogen peroxide to block endogenous peroxidase activity, followed by incubation with 1% BSA for 40 min to block nonspecific binding sites.
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The sections were deparaffinized with xylene.
Paraffin-embedded tissue sections were deparaffinized and hydrated.
Sections were deparaffinized and stained with hematoxylin-eosin (H&E).
Sections were deparaffinized and rehydrated.
Briefly, sections were deparaffinized in xylene.
Sections were deparaffinized 2×5 min in xylene.
Briefly, liver sections were deparaffinized and rehydrated.
Sections were deparaffinized and stained with H&E.
For immunohistochemical studies the sections were deparaffinized and rehydrated.
For immunohistochemical stainings sections were deparaffinized and rehydrated.
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