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Images of TRAP or immune-stained bone sections were captured with a Leica ICC50 camera connected with a Leica DM 750 microscope (Leica Microsystems Heidelberg, Mannheim, Germany) equipped with N Plan objective 5×/-0.12 5×/-0.12laNAobjective 10×/0.25 NA and objective 20×, all from Leica.
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Photomicrographs of sections were captured to perform histomorphometric measurements on the entire area of the tibial subchondral bone with ZEISS Scope A1 (Zeiss, Heidelberg, Germany), Quantitative histomorphometric analysis was conducted in a blinded fashion using the OsteoMeasure XP Software (OsteoMetrics, Inc., Atlanta, USA).
Photomicrographs of the sections were captured using an Olympus BX51 microscope and the application Q Capture 7.0 (Q Imaging, Inc.).
Images of dual-stained sections were captured as stated above.
Images of stained sections were captured on a Zeiss LSM510 laser-scanning confocal microscope.
All digital images of tissue sections were captured using the same microscope magnification.
Histological sections were captured by ZEISS – Axioskope 40 microscope equipped with a CoolSNAP-PRO color camera.
Z-stacks of optical sections were captured on a Leica Sp2 laser scanning confocal microscope.
Serial (z) sections were captured with a 0.25 µm step size.
Fluorescent images of BAT sections were captured by fluorescence microscope (Nikon ECLIPSE E600).
Photomicrographs of the liver sections were captured.
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