The hyoid apparatus has four distinct bone sections, with three joints between these sections.
Additionally, in 2002, Huang et al. [22] determined the vibrating behavior of a dental implant under various surrounding bone conditions using bone sections from hogs and FEA.
Bone sections were grossly assessed for weathering characteristics, which revealed notable differences related to exposure to different temperature regimes and soil types.
The connection is composed of double dog bone sections close to the column face to widen the plastic hinge region; consequently, reducing the resultant equivalent plastic strain.
Growth plate morphology was analyzed by Safranin-O stain on P0.5 and P21 long bone sections.
For static histomorphometry, decalcified implanted bone sections were stained with hematoxylin and eosin in most experiments; in some experiments, undecalcified bone sections were stained with Masson's trichrome and used for analysis.
Immunohistofluorescence on fixed bone sections confirmed TIMP-3 expression in bone lining osteoblasts, endothelial cells as well as in megakaryocytes (Figure 2C).
Decalcified bone sections (10 µm) were cut on a microtome housed in a cryostat at −25°C (Bright's) and flash dried onto glass slides.
The bone sections were immunostained with Tartrate Resistant Acid Phosphatase (TRAP) antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) overnight at 4°C.
Static histomorphometric analysis of non-decalcified bone sections was performed in the distal femur, avoiding the growth plate, as previously described [6].
Bone sections were stained with von Kossa/van Giseon reagent, images were captured using a Nikon eclipse 80i microscope and analyzed using NIS Elements AR 2.30 software (Nikon).
