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For all further steps, the hardened MMA blocks embedding the bone samples were used.
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Blood- or bone marrow samples were used to isolate DNA.
For molecular analysis unfractionated bone marrow samples were used for DNA extraction.
The clinical characteristics of the patients whose bone marrow samples were used in this study are presented in Table 1.
For patients with sufficient material available, pretreatment bone marrow samples were used for DNA and total RNA extraction using TRIzol Life Technologiess, Grand Island, NY, USA) according to the manufacturer's protocol with minor modifications.
Whole goosander pineal glands and 10 mg of each pulverized bone, brain and fish samples were used in the analytical procedure.
Non-amplified RNA from the transduced murine primary bone marrow samples was used for validation with quantitative RT-PCR (qRT-PCR).
Student's t-test for dependent samples was used for the bone mineral parameters.
A day 100 post-stem cell transplant bone marrow sample was used in the cloning process for LCDD-02.
The bone samples were decalcified using 5%% nitric acid for 48 h.
In order to remove any contaminants introduced after death and to extract the collagen fraction, all analysed bone samples were treated using a protocol known as Gelatinization.
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