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Demineralized bone samples were stained with either 0.05 µM DAPI (4' 6'-diamidino-2-phenylindole) overnight, or propidium iodide diluted 1∶1000 (w/v) for 2 h, both in PBS at 4' 6'-diamidino-2-phenylindole
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The remaining samples were stained with Toluidine blue (Fluka 89640, Fluka) for assessment of bone, cartilage, connective tissue, and cells.
These bone sections were stained using Paragon (Paragon C&C; New York, USA) or Giemsa while the subcutaneous samples were stained using Giemsa.
For immunostaining, samples were stained with Alexa Fluor secondary antibodies.
At each point samples were stained with PI, and incorporation was quantified by flow cytometry.
After washing with PBS, samples were stained with secondary antibodies as above.
For exclusion of death cells, samples were stained with Hoechst 33342 (Invitrogen).
To exclude dead cells, the samples were stained with propidium iodide (BD Bioscience, Heidelberg, Germany).
The samples were stained with calcofluor white.
These samples were stained by IF.
Samples were stained as indicated.
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