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Bone samples were scanned in physiological 0.9% NaCl solution.
The bone samples were scanned using a high-resolution SkyScan micro-CT system.
All the bone samples were scanned using a high resolution CBCT (Accuitomo 170; Morita, Kyoto, Japan).
A limitation of the present study is that the bone samples were scanned ex vivo by CBCT.
The bone samples were scanned at a resolution of 9 μm and reconstruction was done using the Sky Scan Nrecon software.
X-ray source was set at 70 kV and 100 mA and trabecular region of bone samples were scanned at a nominal resolution (pixels) of 18 mm.
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For three-dimensional analysis of the teeth and alveolar bone, fixed maxillary samples were scanned at the National Imaging Facility, Centre for Microscopy, Characterisation and Analysis (CMCA), The University of Western Australia, by means of a micro-CT system (SkyScan 1176 in vivo micro-CT, Kontich, Belgium).
For bone mineral density measurement and 3D morphometric analysis, collected femur and spine samples were scanned in a CT (computed tomography) system (Skyscan 1172, Skyscan, Aartlesaar, Belgium).
The samples were scanned using IRBA.
Samples were scanned at 25 °C.
Due to different scanning conditions required to visualize subchondral bone and cartilage, each sample was scanned twice (once for each of the conditions mentioned above).
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