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During the measurements, the bone samples were placed on specimen tray.
The bone samples were placed in this solution and incubated at room temperature for 24 hours and frozen and lyophilized afterwards in a similar manner as the saturated preparations.
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Bone sample was placed perpendicular to the tool for performing both types the drilling.
Bone marrow samples were placed in phosphate-buffered saline (PBS; Biomed, Lublin, Poland) (1 : 1), and 6 mL of this solution was layered over 3 mL of Ficoll-Paque (Stem-cells Technologies, USA).
Bone samples from G. gallus were placed in Cenote Xcolak from December, 2008 to March, 2009, and A. mississippiensis samples were placed from July, 2009 to November, 2009.
The samples were placed in a sample holder, and the sample holder placed in a closed cell with gas connections.
Sintered anode samples were placed upon two sample holders with a span of 3 cm.
Samples were placed in polyethylene bags.
Serum samples were placed in designated microwells.
The samples were placed immediately on ice.
Samples were placed at 4 °C before imaging.
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